Selective and Validated Spectrophotometric Methods for Determination of Rosiglitazone and Pioglitazone with 2, 4-dnp
نویسندگان
چکیده
New, simple and sensitive spectrophotometric methods for the determination of rosiglitazone and pioglitazone have been developed. The method is based on the oxidation of 2, 4-dinitrophenyl hydrazine (2, 4DNP) and coupling of the oxidized product with rosiglitazone and pioglitazone to give intensely colored chromogen. Rosiglitazone and pioglitazone showed maximum absorbance at 421 nm and 480 nm with linearity was observed in the concentration range of 15.0-55μg/ml and 30.0-110.0μg/ml respectively. The relative standard deviations of 0.00903 % for rosiglitazone and 0.65 % for pioglitazone were obtained. The recoveries of rosiglitazone and pioglitazone tablets are in the range 97.846±0.52, 99.49±0.96 respectively. The proposed method is simple, rapid, precise and convenient for the assay of rosiglitazone and pioglitazone in commercial tablet preparations.
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